2013, . Adding 100 µM N,N,N′,N′-tetrakis . 1B). Nuclear Stains - CSH Protocols Single-Molecule Super-Resolution Imaging | Nikon's MicroscopyU TMRE assay ab113852 / TMRM assay ab228569: TMRE is the most popular Abcam mitochondrial membrane dye assay.Ex/Em 549/575 nm. A U251 cells were treated with 25, 50 or 100 nM bufalin for 12 h, and cell apoptosis was observed by confocal microscopy after MitoTracker Red CMX ROS, Annexin V-FITC and Hoechst 33,342 co-staining (n = 3). L7528 LysoTracker® Red DND-99 577 590 NA L12492 LysoTracker® Deep Red 647 668 NA L7533 LysoSensor™ Blue DND-167 373 425 5.1 L7535 LysoSensor™ Green DND-189 443 505 5.2 L7534 LysoSensor™ Green DND-153 442 505 7.5 L7545 LysoSensor™ Yellow/Blue DND-160 (PDMPO) 329, 384 ‡ 440, 540 ‡ 4.2 Mitotracker Assay | Thermo Fisher | Bioz Excitation: 644 nm Emission: 665 nm Background: MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety Wash the cells with PBS and resuspend the cells in 100 μL of 1X annexin-binding buffer. Product Description. Tamr cells were subjected to assess PDF MitoTracker® Mitochondrion-Selective Probes Product name MitoTracker® Red CMXRos *special packaging* Company/undertaking identification 24 hour Emergency Response: 866-536-0631 301-431-8585 Outside of the U.S. ++1-301-431-8585 . Rab5a is an early endosome marker. The other two, MitoTracker-Red and MitoTracker-Orange, showed multiple fluorescence lifetime populations in their phasor plots (Supporting Figure S1). To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria. Structure-specific small molecule dyes . Directions for Use: Each vial of MitoTracker® Red CMXRos contains 50 μg lyophilized solid. Principle of the Mitophagy Detection Kit using Dojindo's unique Mtphagy and Lyso dyes. principles of all other metabolites may play a mitochondrion. . Coronary arterial endothelial cells (CAECs) were treated with resveratrol (10 mol/l for 48 h) in Isolated myofibers were stained with mitotracker deep red (M2246, Thermo Fisher) and NucBlue (R37605, Thermo Fisher) in DMEM for 30 min. Influence of AEDG and KE Peptides on Mitochondrial Staining and the ... After 240 min of reperfusion, we observed the MitoTracker Orange-labeled mitochondria distributed among the cryosectioned liver parenchyma. On depolar-ization, the orange-red punctate staining is replaced by diffuse green monomer fluorescence. PDF MitoTracker Deep Red FM - Cell Signaling Technology To 1 mL of cells, add 4 μL of 10 μM MitoTracker® Red working solution from the above step and stain for 30 minutes at 37°C in an atmosphere of 5% CO 2. The nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue fluorescence), while the mitochondria and actin cytoskeleton were stained with MitoTracker Red (red fluorescence) and a phalloidin derivative (green fluorescence), respectively. PPT Slide 1 are red. Positively and negatively charged amino acids are shown in orange . Lung Cell. between MitoTracker Green and MitoTracker Red, as expected, but not between MitoTracker Green and the Red-to-Green ratio (Fig. Reaction-based fluorescent sensor for investigating mobile Zn2+ in ... MitoTracker Deep Red 633 is really a red fluorescence which Posted on April 26, 2013 MitoTracker Deep Red 633 is just a red fluorescence which iswell settled fromthe green fluorescence of MitoTracker Green FM, thus it is designed for multicolor labeling experiments. Mitochondrial imaging in live or fixed tissues using a luminescent ... In principle, however, PK Mito dyes should generally reduce the photodamage during image acquisitions under a variety of fluorescence microscopies. ZEISS Lattice Lightsheet 7 PDF Safety Data Sheet MitoTracker® red (Mitochondria), Alexa Fluor® 568 (F-actin), and SYTOX® Orange (Nucleus) were imaged . Population without losing membrane potential most likely plays a drop of fluorescence. Sign In - CSH Protocols Due to the transport of electrons in the respiratory chain, the inner membrane is charged. Assigning mitochondrial localization of dual localized proteins ... - eLife Introduction . Anna Dubiel Principles of fluorescentprobes Warsaw, 17.05.2011. . This technique uses the principle of capturing continuous images of motile sperm from a microscopic field and converts images into video images with different acquisition rates (frames s-1, Hz). A photostable fluorescent marker for the superresolution live ... - PNAS Brad Chazotte; Cold Spring Harb Protoc; 2011; doi: 10.1101/pdb.prot5648 . GFP β1-10 sequence encoding the first ten beta strands of GFP has been integrated into the mitochondrial genome under the control of the ATP6 promoter. MitoTracker Deep Red 633 is really a red fluorescence which Coord Chem Rev 173, 133-165 (1998). - mtSOX Deep Red - Mitochondrial Superoxide Detection #MT14 After recording an initial T1 measurement, 1, 5, and 10 CCCP as well as SOD (1000 U/ml) and CAT (600 U/ml) were tested on the same samples. Table 1 Contents and storage Product Catalog No. Labeling mitochondria with MitoTracker dyes - PubMed Am. Cells were then treated with Mitotracker red, fixed and analyzed for DNM1L expression and Mitotracker signal by confocal microscopy. Indicators for Ca2+ Fura Red λabs=473, 436nm λem=670, . Photostability comparison between TPE-TETRAD@biotin AIE NPs (red) and MitoTracker Green (green) in HeLa cervical cancer cells with increasing number of laser scans at 488 nm. The cells were washed twice with phosphate buffered saline and stained with 0. First, HeLa cells were stained with each dye un-der appropriate conditions and then fixed with 4% para-formaldehyde (15 min) followed by washing with a PBS buffer.